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3. School of Pharmacy Home - School of Pharmacy
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6. Organization of the class I region of the bovine major histocompatibility ...
12 apr 2006 · ... order and chromosomal location through PCR, novel BAC end ... The BoLA class I region is immunologically important for disease ...
The major histocompatibility complex (MHC) is a genomic region containing genes of immunomodulatory importance. MHC class I genes encode cell-surface glycoproteins that present peptides to circulating T cells, playing a key role in recognition of self and non-self. Studies of MHC loci in vertebrates have examined levels of polymorphism and molecular evolutionary processes generating diversity. The bovine MHC (BoLA) has been associated with disease susceptibility, resistance and progression. To delineate mechanisms by which MHC class I genes evolved to function optimally in a species like cattle, it is necessary to study genomic organization of BoLA to define gene content, and investigate characteristics of expressed class I molecules. This study describes development of a physical map of BoLA class I region derived from screening two BAC libraries, isolating positive clones and confirming gene content, order and chromosomal location through PCR, novel BAC end sequencing techniques, and selected BAC shotgun cloning and/or sequencing and FISH analysis. To date, this is the most complete ordered BAC array encompassing the BoLA class I region from the class III boundary to the extended class I region. Characterization of a frameshift allele exhibiting trans-species polymorphism in Bos and Bison by flow cytometry, real-time RT-PCR, 1D and 2D gel analysis is also described. This frameshift allele encodes an early termination signal within the antigen recognition site (ARS) of exon...
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Prediction of peptide interactions with bovine MHC Class II BoLA-DRB3 molecules. ... EL_Rank: Rank of the predicted eluted ligand likelihood score compared ...
Prediction of peptide interactions with bovine MHC Class II BoLA-DRB3 molecules
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